alpha smooth muscle actin marker

We have examined the expression of smooth muscle alpha-actin in hair follicles in situ, and in hair follicle dermal cells in culture by means of immunohistochemistry. Alpha Smooth Muscle Actin is frequently used as a marker of smooth muscle differentiation and has a theoretical molecular weight of 42 kDa. alpha-smooth muscle Actin is a blood vessel marker in the brain. alpha sma fibroblast marker. smooth muscle myosin heavy chain (sm-mhc) and alpha smooth muscle actin (-sma) for immunodetection of adventitial and. : 449 004. Scale bars represents 20 m. Alpha smooth muscle actin antibody (ab5694) stains smooth muscle cells in vessel walls, gut wall, and myometrium. Aliannah Hope Simms's Bio, Age. Naati Fakatava, Hiromi Mitarai, Asuka Yuda, Akira Haraguchi, Hiroko Wada, Daigaku Hasegawa, Hidefumi Maeda, Naohisa Wada. . The secondary antibody ImmPRESS HRP anti-mouse IgG was incubated for 20 min at RT. Data are represented as mean SD. ARG63621 anti-alpha smooth muscle Actin antibody ICC/IF image Immunofluorescence: Paraformaldehyde fixed HepG2 cells permeabilized with 0.15% Triton. ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] IHC-P image Rationale: Airway smooth muscle (SM) . Skalli O, Pelte MF, Peclet MC, Gabbiani G, Gugliotta P, Bussolati G, Ravazzola M, Orci L. Alpha-smooth muscle actin, a differentiation marker of smooth muscle cells, is present in microfilamentous bundles of pericytes. Tested applications Suitable for: WB, IHC-P more details Species reactivity In our study, SM1, SM2, and SMemb were all positive in the normal media, as is the case in human teenagers . Here we show that actin fibers decorated with h1 CaP remain stable, whereas SM22 alpha-decorated actin bundles undergo rapid reorganization into podosomes within 30 min of PDBu exposure. 21-23 smmhc is a structural component (200 kd) unique to smooth muscle cells, which functions within the hexagonal array of the thick-thin filament contractile apparatus. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). The main cells involved in fibrosis are pancreatic stellate cells (PSCs), which activate alpha smooth muscle actin (SMA), which is considered to be the best-known fibrosis marker. Request PDF | Distinct patterns of responses in endothelial cells and smooth muscle cells following vascular injury | Vascular smooth muscle cells (SMCs) are heterogeneous, and their differential . In contrast, in . Match Criteria: Product Name, Keyword. Bright field pictures of the b) ODECs, c) HUVECs, d) ODMCs, and e) VSMCs. . The aim of the study was to evaluate the expression of the SMA in patients with PDAC and CP as the possible differentiation marker. Cat. Monoclonal Antibody for studying ACTA2 (alpha actin, smooth muscle). continental double fighter iii 700c; what are the 3 types of embroidery needles? -Smooth muscle actin (-SMA) is used as a marker for a subset of activated fibrogenic cells, myofibroblasts, which are regarded as important effector cells of tissue fibrogenesis. DAPI (blue) for nuclear staining. [8] References [ edit] Compare Product No. calponin and smmhc are two antibodies that are more specific for mecs. Dermal papilla cells within all types of follicles did not express the antigen. Haeberle JR, Trybus KM, Hemric ME, Warshaw DM. In adult mice, different skeletal stem/progenitor cells (SSCs) are responsible for the repair of distinct bone injuries. J Biol Chem 1992;267: 23001 -23006. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). There is a chance that this product will detect other actin family members. In SMC and pericytes, alpha-sm actin was localized in microfilament bundles, strengthening the assumption that it is the functional isoform in these cell types and supporting the assumptions that each cell types exert contractile functions. With the progression of atherosclerosis, the expression of SM2 first decreases in the smooth-muscle cells in the intima followed by a decrease in the expression of SM1, whereas smooth-muscle -actin is well preserved . alpha-Smooth muscle (alpha-sm) actin, an isoform typical of smooth muscle cells (SMC) and present in high amounts in vascular SMC, was demonstrated in the cytoplasm of pericytes of various rat and human organs by means of immunocytochemistry at the electron microscopic level. Although several markers of SM have been studied in asthma, myosin, being the molecular motor that drives contraction, is the marker of choice. The pulsatile pressure produced by the heart causes a cyclic distention of the aorta. Myoepithelial cells in breast and salivary gland are also stained. This 1A4 antibody is specific for the alpha form of muscle actin only, which is expressed by smooth muscle cells of blood vessels, myofibroblasts, and myoepithelial cells. Hide. Unlabelled sample (green) was used as a control. The primary antibody anti-alpha-Smooth Muscle Actin mouse monoclonal, 1A4/ASM-1 (Cat. The immunoreactivity of a range of vascular and nonvascular smooth muscle tumours, rhabdomyosarcomas, and nonmyoid lesions has been examined with the use of a monoclonal antibody to smooth muscle . Such a regional marker of meso- from those of other vertebrates, it was important to deter- derm should fulfil four criteria: (i) it should be expressed mine the specificity of the -SM actin antibody used in this in ventrolateral mesoderm derivatives, (ii) it should be acti- study (Skalli et al., 1986). [Google Scholar] Fujimoto T, Singer SJ. Alphasmooth muscle actin as a marker for soft tissue tumours: A comparison with desmin . ab5694 reacts with tumors arising from smooth muscles and myoepithelial cells. The cross reactivity has not been experimentally determined. Validated for WB, IP, IF. It is proposed that this monoclonal antibody to alphasmooth muscle actin is a useful addition to the panel of reagents used for the characterization of soft tissue proliferations and tumours. 690001) was diluted in PBS (antibody concentration 400 ng/ml) and incubated at 4C over-night. Amount: 100 l. Four CAF cell lines were isolated from patients with luminal breast cancer (lumBC) and classified according to the presence of -SMA protein. Scale bars represent 20 m. Highly specific and rigorously validated in-house, -Smooth Muscle Actin (1A4) Mouse Monoclonal Antibody (IF Formulated) (CST #48938) is ready to ship. Masumoto, K.; Suita, S.; Taguchi, T., 2003: The occurrence of unusual smooth muscle bundles expressing alpha-smooth muscle actin in human intestinal atresia However, in culture a large . Changes in the arterial wall, associated with vascular diseases such as atherosclerosis and hypertension, strongly influence this process. Alpha-smooth muscle actin (-SMA), transgelin (TAGLN), Myosin Heavy Chain 11 smooth muscle (Myh11), cytokeratin (CK)-18, S100 protein A4 (S100A4), calmodulin 2 (CALM2), Intercellular Adhesion Molecule (ICAM)1, Krppel-like factor 4 (KLF4). alpha-Smooth muscle (alpha-sm) actin, an isoform typical of smooth muscle cells (SMC) and present in high amounts in vascular SMC, was demonstrated in the . J Histochem Cytochem. Smooth muscle alpha-actin was present in the dermal sheath component of rat vibrissa, rat pelage and human follicles. Abstract The immunoreactivity of a range of vascular and nonvascular smooth muscle tumours, rhabdomyosarcomas, . 1989 Mar; 37 (3):315-321. In the absence of well-characterized markers, -smooth muscle actin (SMA) is often used to identify pathologic fibroblasts, and some authors have equated SMA (+) cells with contractile myofibroblasts and proposed that these cells are the major source of ECM. No. All Photos (2) Anti-Actin-2, N-Terminal antibody produced in rabbit. Definition / general Actin is a 43000 kDa ubiquitous protein found in all cells Actins are involved in cell motility (alpha, smooth muscle) and the maintenance of the cytoskeleton (beta and gamma, all cells) Antibodies to alpha smooth muscle actin do not detect the other actin isoforms Essential features Involved in cell motility single unit smooth muscle (tonic) - generate their own level of rhythmic contraction - found in: uterus, git, ureter and urinary bladder - activity modulated by postganglionic parasympathetic neurons release ach binds to m3 muscarinic ach receptors (machr) - postganglionic sympathetic neurons release norepinephrine, which binds to alpha 1 and Actin alpha 2, smooth muscle, a transforming growth factor-1-induced factor, regulates collagen production in human periodontal ligament cells via Smad2/3 pathway. The effect of polyamidoamine third-generation dendrimer (PAMAM) on -smooth muscle actin expression in treated and control wounds. Alpha-smooth muscle actin (-SMA) was examined immunohistochemically in intratumoral CAFs of nonmetastatic breast cancers and correlated with clinicopathological data. Guinea pig polyclonal antiserum. custom hamburgers near me; best 15 inch off-road tires; integrated project delivery method pros and cons. ACTA2 (commonly referred to as alpha-smooth muscle actin or -SMA) is often used as a marker of myofibroblast formation. the use of vascular smooth muscle cell markers, e.g. 24 calponin, a 34-kd polypeptide, modulates actomyosin adenosine triphosphatase (atpase) activity in the Alpha-Smooth Muscle Actin Antibodies Antibodies that detect Alpha-Smooth Muscle Actin can be used in several scientific applications, including Immunohistochemistry, Western Blot, Immunocytochemistry, Flow Cytometry and ELISA. Blue Prestained Protein Marker, Broad Range (11-250 kDa): . Alpha-smooth muscle actin (alpha-SMA) is the actin isoform that predominates within vascular smooth-muscle cells and plays an important role in fibrogenesis. Calponin and SM22 alpha are major components of differentiated smooth muscle and potential regulators of actin cytoskeleton interactions. Clonality Application Species Reactivity Citations SDS; SAB5600065: RM253, monoclonal recombinant monoclonal: IHC: human: Expand. It is a member of the Actin family. No. It is abundant in vascular and visceral smooth muscle cells. RT-PCR analysis of the effect of M1 macrophages on VSMCs phenotype markers. -Smooth muscle actin is an inconsistent marker of fibroblasts responsible for force-dependent TGF activation or collagen production across multiple models of organ fibrosis Kai-Hui Sun , Yongen Chang , Nilgun I. Reed , and Dean Sheppard 01 May 2016 https://doi.org/10.1152/ajplung.00350.2015 This is the final version - click for previous version She was born with a uncommon type of muscular dystrophy named Titin muscular dystrophy , which has only ever been identified in grownups. Synonym(s): alpha-Actin-2, human alpha smooth muscle Actin. This protein is reported to have a cytoplasmic cellular localization. LepR+Adiponectin+ bone marrow SSCs are responsible for steady-state osteogenesis and drill injury repair while Gli1+ periosteal SSCs are primarily responsible for bicortical fracture repair and bone marrow stromal cell regeneration at fracture sites. The effects of smooth muscle caldesmon on actin filament motility. Myofibroblasts are. Upon arterial injury, expression of SMA and other structural proteins decreases and SMCs acquire a pro-migratory and proliferative phenotype. Cited in 28 publications. bell panovision shield; pirelli brand ambassador; grasp neo camo waterproof rain boot bogs; alpha-Smooth muscle (alpha-sm) actin, an isoform typical of smooth muscle cells (SMC) and present in high amounts in vascular SMC, was demonstrated in the cytoplasm of pericytes of various rat and human organs by means of immunocytochemistry at the electron microscopic level. . Smooth muscle alpha-actin (SMA) is a marker for the contractile, non-proliferative phenotype of adult smooth muscle cells (SMCs). The human protein, encoded by the gene ACTA2, is 377 amino acid residues long and has a mass of 42,009 daltons.

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