vinculin antibody mouse

Reactivity: Human, Mouse and more. 6 B, panels c-f), attesting that the drugs were active and probably affected the stability of focal adhesion complexes. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. Anti-Vinculin antibody [EPR8185] (ab129002) Research with confidence - consistent and reproducible results with every batch Long-term and scalable supply - powered by recombinant technology for fast production Success from the first experiment - confirmed specificity through extensive validation Show Size & Price. 293T, PC-12, Mouse heart, Mouse skeletal muscle Cellular location Cell junction, Cell membrane, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, adherens junction, cytoskeleton, focal adhesion . Our Vinculin Antibodies can be used in a variety of model species: Amphibian, Bovine, Canine, Chicken, Human, Mouse, Rat, Turkey. The cells were blocked with 10% goat serum. Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions. Biochem/physiol Actions Vinculin functions as one of several interacting proteins involved in anchoring F-actin to the membrane. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. The anti-integrin 3 antibody used in immunoprecipitation experiments is a mouse polyclonal antibody (GPIIIa), whereas the anti-3 antibody used for Western blotting is a rabbit antibody (CD61). Thrombogenicity remains a major issue in cardiovascular implants (CVIs). -Tubulin was used as a loading control. Alomone Labs p2x7 antibodies P2x7 Antibodies, supplied by Alomone Labs, used in various techniques. Cited in 11 publication(s). Use the list below to choose the Vinculin Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Vinculin belongs to the vinculin/alpha-catenin family. Anti-Vinculin antibody is purified via Protein A purification. Vinculin Polyclonal Antibody, AbBy Fluor 405 Conjugated www.biossusa.com [email protected] 800.501.7654 [DOMESTIC] +1.781.569.5821 [INTERNATIONAL] DATASHEET. Immunohistochemical analysis of paraffin-embedded mouse spleen using Vinculin . ABclonal provides trial size antibody samples for target detection. B16F10 mouse melanoma cells, vinculin-deficient mouse embryonic fibroblast . Anti-Vinculin Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. Bioz Stars score: 97/100, based on 5 PubMed citations. Peroxidase-conjugated anti-mouse and anti-rabbit IgG (1:10,000) were used as secondary antibodies. MAB68961. Invitrogen Anti-Vinculin Recombinant Monoclonal (3J7P3), Catalog # MA5-42795. Spontaneous escapers gen-conditional oncogenic Kras mouse model of pancreatic ductal erated tumours with high penetrance upon . Embryos were lysed in NP-40 buffer and immunoprecipitated using the monoclonal antibody to C-cadherin 6B6. Description: The monoclonal antibody 7F9 (VIIF9) recognizes human, mouse, rat, and avian vinculin and its alternatively spliced isoform, metavinculin. Secondary. Validated for WB, IHC, IHC, F. Available in 2 sizes. Independently reviewed in 5 review(s) Protein A-Sepharose 4B, glutathione-agarose beads, human plasma fibronectin, and the mouse monoclonal -vinculin were purchased from Sigma. Validations None available Applications None available Reactivity Homo sapiens (Human) Mus musculus (House mouse) Rattus norvegicus (Rat) Mechanical interactions between a cell and its environment regulate migration, contractility, gene expression, and cell fate. Store at -20C as received. Blots were blocked in 5% BSA in Tris-buffered saline (TBS)/0.1% Tween 20 (TTBS) for 1 hour at ambient temperature before incubating overnight with 1:1,000 dilution of primary antibody in TTBS/0.5% BSA at 4C. GTX113294 Reference 7 3 Datasheet File See all Vinculin products APPLICATION Application Note *Optimal dilutions/concentrations should be determined by the researcher. Cited in 313 publications. Packaging, Storage & Formulations Alternate Names for Vinculin Antibody (hVIN-1) CMD1W CMH15 metavinculin It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. VCL (Vinculin; from the Latin vinculum denoting a union or connection) is a 115-120 kDa cytoplasmic member of the vinculin/ alpha -catenin family of proteins. Vinculin antibody [N1N3] Cat No. Optical density of bands was quantified by ImageJ software. Lane 5: PC-12 whole cell lysate. Vinculin Monoclonal Antibody (VLN01) Product Details Size 500 L Species Reactivity Human, Mouse, Non-human primate . FREE Shipping A cytoskeletal protein associated with cell-cell and cell-matrix interactions. Independently of cell spreading, integrin binding and focal adhesion assembly resulted in rapid sevenfold increases in . Mouse Rat Rabbit Predicted Cross Reactive Species . 2.5. Relative variation for the intensity of specific target proteins to vinculin was compared between treated and control samples. AF6896. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. Journal Cover Articles. At least three isoforms of Vinculin are known to exist. Two bacteriophage M13 vinculin Antibody (H-10) is a mouse monoclonal IgG 2a , cited in 144 publications, provided at 200 g/ml raised against amino acids 1-300 of vinculin of human origin Anti-vinculin Antibody (H-10) is recommended for detection of vinculin of mouse, rat and human origin by WB, IP, IF, IHC (P) and ELISA Lane 6: HepG2 whole cell lysate. Purity/Specificity. Replacement to Abcam, Santa Cruz, Sigma and CST antibody. 100% Guaranteed. Please contact Technical Support if you have any questions. Highly specific and rigorously validated in-house, Vinculin (E1E9V) XP Rabbit Monoclonal Antibody (CST #13901) is ready to ship. The present disclosure provides, in part, a priming and boosting vector-based platform to develop vaccines against pathogens that is tailored to elicit a broad T cell response targeting conserved vira Blots were washed three times with TTBS for 10 minutes, for a total of 30 minutes. Lysates/proteins at 20 g per lane. It may also play important roles in cell morphology and locomotion. The immunoprecipitates were immunoblotted with the polyclonal anti-aN-catenin antibody (CME). Vinculin is involved in the anchoring of F-actin to the membrane and the regulation of E-cadherin expression. Host: Rabbit. Sample: Paraffin-embedded rat testis. Vinculin (E1E9V, Cell Signaling expresses the codon-optimized Flpo recombinase under . Cytokine assay Vinculin antibody can be used for Western Blot, ELISA, and other antibody applications. Lane 2: A431 whole cell lysate. Staining with K20, an antibody that detects all forms of 1 integrin, confirmed that the results with 12G10 were due to conformational changes in integrin and not due to increased integrin expression . SDS CoA References Data Sheet CP74 Enter the email address you signed up with and we'll email you a reset link. This Anti-Vinculin Mouse mAb (V284 or VLN01) is validated for use in Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Vinculin. vinculin antibody (7f9) is a high quality monoclonal vinculin antibody (also designated metavinculin antibody or vcl antibody) suitable for the detection of the vinculin protein of human, mouse, rat and avian origin. Vinculin (1:2000, Sigma-Aldrich, V913) was used as a housekeeping protein to normalize the loading variations in protein concentration. This antibody reacts with Human, Mouse, Rat samples. All other applications and species not yet tested. Vinculin antibody is human, mouse, rat, rabbit and chicken reactive. Human (3) Calculated MW 124 kDa. The VCL gene is ubiquitously expressed and generates a series of globular domains whose N-and C-terminal globular regions self-associate to generate an inactive molecule. Specific conditions for reactivity should be optimized by the end user. The protein consists of 1066 amino acid residues and. 2 Images. Source Monoclonal Mouse IgG 2A Clone # 728526 Purification Protein A or G purified from hybridoma culture supernatant Immunogen E. coli -derived recombinant human Vinculin Lys1020-Gln1134 page of 1. 2 Images. Protocol Specificity / Sensitivity Vinculin Antibody detects endogenous levels of total vinculin protein. Clonality: Polyclonal. Applications: WB, IHC and more. Purity/Specificity Vinculin belongs to the vinculin/alpha-catenin family. Vinculin (1 lg) was adsorbed on non-adjacent wells of a 96-well polystyrene mictotitre plate, non-specic binding sites were blocked with 1.0% (w}v) BSA, and then 10"" phage particles in wash buer [PBS (pH 7.5)}0.1% Tween-20}0.1% BSA] were incubated for 4 hat room temperature. Buy mouse monoclonal (V284) antibody to Vinculin (A281893). Vesicles/particles are indicated by yellow arrows, white arrows point to focal adhesion and green arrows point to vinculin in the nucleus. Cells were stained using the NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and . This antibody reacts with human, mouse, rat, amphibian, bovine, canine, chicken, turkey. Instrument default settings were as follows: stacking and separation at 475 V for 30 min; blocking reagent for 5 min, primary and secondary antibodies for 30 min each; luminol/peroxide chemiluminescence detection for ~15 min. . Apply. Anti-Vinculin Mouse mAb (V284 or VLN01) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. Supplied as 100 L purified antibody (1 mg/mL). Vinculin was detected in immunocytochemical section of human mammary cancer cell. Anti-Vinculin antibody, Mouse monoclonal has been used in immunoblotting immunofluorescence staining immunocytochemistry immunohistochemistry and enzyme linked immunosorbent assay (ELISA). Do not aliquot the antibody. Vinculin binding to -catenin is not required for the formation of cell-cell junctions but stabilizes junctional -catenin Stable for 12 months from date of receipt. Selected Publications [20] Single-cell analysis of embryoids reveals lineage diversification roadmaps of early human development Yi Zheng, Robin Zhexuan Yan, Shiyu Sun, Mutsumi Kobayashi, Lifeng Xiang, Ran Yang, Alexander Goedel, Yu Kang, Xuefeng Xue, Sajedeh Nasr Esfahani, Yue Liu, Agnes M. Resto Irizarry, Weisheng Wu, Yunxiu Li, Weizhi Ji, Yuyu Niu, Kenneth R. Chien . ( Note ) Positive Control NT2D1 , Mouse brain , *HeLa Predict Reactivity ZERO BIAS - scores, article reviews, protocol conditions and more - Find MSDS or SDS, a COA, data sheets and more information. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. ICC/IF, IHC. The Cadherin-based adherens junctions anchor via. Vinculin antibody is purified via Protein A prification. Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell-extracellular matrix adherens-type junctions. Back to Search Results.

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